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KMID : 0604520200460040349
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Journal of the Society of Cosmetic Scientists of Korea
2020 Volume.46 No. 4 p.349 ~ p.360
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Biotransformation of Ginsenoside Rd from Red Ginseng Saponin using Commercial β-glucanase
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Kang Hye-Jung
Lee Jong-Woo
Park Tae-Woo
Park Hye-Youn
Park Jun-Seong
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Abstract
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Bio-conversion manufacturing technology has been developed to produce ginsenoside Rd which is increasingly in demand as a cosmetic material due to various possibilities related to improving skin function. In order to convert ginsenoside Rb1 which is contained in red ginseng saponin (RGS) into Rd, several commercial enzymes were tested.
Viscoflow MG was found to be the most efficient. In order to optimize the conversion of RGS to ginsenoside Rd by enzymatic transition was carried out using response surface methodology (RSM) based on Box-Behnken design (BBD).
The main independent variables were RGS concentration, enzyme concentration, and reaction time. Conversion of ginsenoside Rd was performed under 17 conditions selected according to BBD model and optimization conditions were analyzed. The concentration of the converted ginsenoside Rd ranged from 0.3113 g/L to 0.5277 g/L, and the highest production volume was obtained under condition of reacting 2% RGS and 1.25% enzyme for 13.5 hours. Consequently, RGS concentration, enzyme concentration which is 0.05 less than p-value and among the interactions between the independent variables, the interaction between enzyme concentration and reaction time was confirmed to be the most influential.
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KEYWORD
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red ginseng extract, ginsenoside Rd, bioconversion, β-glucanase, RSM
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